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KMID : 1011620170330040380
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Korean Journal of Food and Cookey Science
2017 Volume.33 No. 4 p.380 ~ p.386
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Methods for Viable Cell Counts and Community Analysis of Microbial Populations on Broccoli
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Kim Min-Su
Park Eun-Jin
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Abstract
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This study examined methods for viable cell counts and microbial analysis of leaf surfaces of broccoli. Methods: For analysis of microbial cell counts on the leaf surfaces of vegetables including broccoli, we performed physical homogenization using a stomacher with cell separation using ultrasound. Results: In the comparison of cell separations using a stomacher and ultrasound, no significant differences in total bacterial and fungal cells were found in all treated samples (p>0.05). The amount of megagenomic DNAs of broccoli leaf-associated microbiota were not significantly different over time in the ultrasonic wave (p>0.05). The number of sequences assigned to bacteria was different in two different primer sets. There was no significant difference in total cell counts of bacteria and fungi on broccoli leaves collected from 10 local farms and 10 grocery stores in Seoul and Jeju Island. Conclusion: These results suggest that ultrasonic treatment for 5-10 min is a suitable method to determine viable cell counts and community analysis of broccoli.
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KEYWORD
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broccoli, Brassica oleracea L. Italica group, ultrasound, microbial cell count, 16S rRNA gene amplification
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